ABSTRACT
Background: The use of novel materials as an artificial extracellular matrix for stem cell growth is a current strategy of increasing interest for regenerative medicine. Here, we prepare thermal-remolded membrane scaffolds from poly(3-hydroxybutyrate) grafted with 2-amino-ethyl methacrylate hydrochloride. However, it is unclear whether these membranes are useful for tissue engineering. Results: The mechanical properties, tribology, and morphology of the dense membranes were assessed. The results show that tensile strain at break and roughness of the compressed membrane decrease with increasing graft degree. Moreover, graft copolymer membranes showed lower resistance to scratching, greater degree of swelling and higher brittleness than un-grafted P(3HB) films. Thus, it effectively supports the growth of dermal fibroblast, as demonstrated by epifluorescence microscopy. Conclusions: It is concluded that the developed membrane can be properly used in is the restoration of skin tissue. How to cite: González-Torres M, Sánchez-Sánchez R, Solís-Rosales SG, et al. Poly(3-hydroxybutyrate) graft copolymer dense membranes for human mesenchymal stem cell growth.
Subject(s)
Mesenchymal Stem Cells/physiology , Membranes, Artificial , Temperature , Regenerative Medicine , GrowthABSTRACT
Los dientes pueden presentar trastornos originados por factores externos o hereditarios que afectan su forma. Dens in dente es una anomalía durante el desarrollo que resulta en la invaginación del esmalte y dentina, en la cara lingual o vestibular, que puede extenderse profundamente dentro de la raíz. El talón cuspídeo es caracterizado por la proyección de una cúspide accesoria en el cíngulo de los órganos dentarios anteriores y la superficie oclusal de los posteriores. Se presenta el caso de una niña de 7 años de edad, con dens in dente y talón cuspídeo en el incisivo central superior derecho que provoca interferencia oclusal prematura, sin sintomatología. Se realizó desgaste gradual del talón cuspídeo, con aplicación de fluoruro de sodio. El diagnóstico temprano de anomalías dentales en pacientes pediátricos es la clave para un tratamiento conservador y preventivo para el adecuado funcionamiento masticatorio y estético del paciente (AU)
Teeth may have disorders caused by external or hereditary factors that affect their shape. Dens invaginatus is an anomaly during development that results in invagination of the enamel and dentin, lingual or vestibular surface, which can extend deeply into the root. Talon cusp is characterized by the projection of an accessory cusp in the cingulate area in anterior and occlusal aspects of the posterior teeth. We present a case of a 7 year old girl with dens invaginatus and talon cusp on the maxillary central incisor that causes premature occlusal interference, without symptomatology. The treatment employed were gradual wear of cuspal talon with several applications of sodium fluoride. Early diagnosis in pediatric patients is the key to a conservative and preventive treatment for a proper masticatory and aesthetic functioning of the patient (AU)